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Does the measurement work in pigmented animals?

Skin pigmentation does not affect the measurement in a negative fashion. As the melanin absorbs large amounts of blue and green light the overall signal measured by the tGFR Monitor will be lower. However as reflecting excitation light, skin auto fluorescence, and scatter light from the outside is also absorbed, the background signal drops at the same time. Therefore, the background to peak ratio stays the same. Moreover, as the reflections and scatter are the reason for movement artifacts in the datasets, the measurements get much smoother when measuring on dark skin. For that reason, measurement quality in pigmented skin is generally even better in respect of signal to noise.