Gain Access to exclusive preclinical content.
Complete the form below to gain access to mattis aliquet nunc aliquam tempor. We’ll assign a user name and password, then send you a link. Thanks!

Get Access Register

You must log in to access content on this page. Or click here to request access.
How can I cite the TGFR Technique in my publication?

Please adjust according to the species, FITC-Sinistrin amount, measurement time and evaluation procedure:

Transdermal GFR measurement was done as described previously1,2. In short, mice/rats were anesthetized with isoflurane and a miniaturized fluorescence detector built from two light-emitting diodes, a photodiode and a battery (MediBeacon GmbH, Mannheim, Germany) was mounted via a double-sided adhesive tape onto the shaved and depilated animals' back. Prior to the intravenous injection of 75 or 50 mg/kg FITC-sinistrin (MediBeacon GmbH, Mannheim, Germany), the skin's background signal was recorded for 5 min. For the duration of recording (~1.5/2.5 h) each animal was conscious and kept in a single cage. After removing the device data were analyzed using MB Lab/MB Studio software (MediBeacon GmbH, Mannheim, Germany). The GFR [μl/min or µL/min/b.w.] was calculated from the decrease of fluorescence intensity over time (i.e., plasma half-life of FITC-sinistrin) using a one-/two-/three4- compartment model, the animals’ body weight and an empirical conversion factor1,3.

  1. Schreiber A, Shulhevich Y, Geraci S, Hesser J, Stsepankou D, Neudecker S, Koenig S, Heinrich R, Hoecklin F, Pill J, Friedemann J, Gretz N, Schock-Kusch D. Transcutaneous measurement of renal function in conscious mice. Am J Physiol Renal Physiol. 2012;303(5):F783-F788. doi:10.1152/ajprenal.00279.2012
    (cite for procedure and mouse conversion factor)
  1. Scarfe L, Schock-Kusch D, Ressel L, Friedemann J, Shulhevich Y, Murray P, Wilm B, de Caestecker M. Transdermal Measurement of Glomerular Filtration Rate in Mice. Vis. Exp. (140), e58520, doi:10.3791/58520 (2018). (cite for procedure)
  1. Schock-Kusch D, Sadick M, Henninger N, Kraenzlin B, Claus G, Kloetzer H-M, Weiß C, Pill J, Gretz N. Transcutaneous measurement of glomerular filtration rate using FITC-sinistrin in rats, Nephrology Dialysis Transplantation, Volume 24, Issue 10, October 2009, Pages 2997–3001, (cite for rat conversion factor)
  1. Friedemann J, Heinrich R, Shulhevich Y, Raedle M, William-Olsson L, Pill J, Schock-Kusch D. Improved kinetic model for the transcutaneous measurement of glomerular filtration rate in experimental animals. Kidney Int. 2016 Dec;90(6):1377-1385. doi: 10.1016/j.kint.2016.07.024. Epub 2016 Sep 21. PMID: 27665115. (cite for 3 compartment model used in MB Studio software)