Please adjust according to the species, FITC-Sinistrin amount, measurement time and evaluation procedure:

Transdermal GFR measurement was done as described previously^1,2. In short, mice/rats were anesthetized with isoflurane and a miniaturized fluorescence detector built from two light-emitting diodes, a photodiode and a battery (MediBeacon GmbH, Mannheim, Germany) was mounted via a double-sided adhesive tape onto the shaved and depilated animals' back. Prior to the intravenous injection of 75 or 50 mg/kg FITC-sinistrin (MediBeacon GmbH, Mannheim, Germany), the skin's background signal was recorded for 5 min. For the duration of recording (~1.5/2.5 h) each animal was conscious and kept in a single cage. After removing the device data were analyzed using MB Lab/MB Studio software (MediBeacon GmbH, Mannheim, Germany). The GFR [μl/min or µL/min/b.w.] was calculated from the decrease of fluorescence intensity over time (i.e., plasma half-life of FITC-sinistrin) using a one-/two-/three⁴- compartment model, the animals’ body weight and an empirical conversion factor^1,3.

1. Schreiber A, Shulhevich Y, Geraci S, Hesser J, Stsepankou D, Neudecker S, Koenig S, Heinrich R, Hoecklin F, Pill J, Friedemann J, Gretz N, Schock-Kusch D. Transcutaneous measurement of renal function in conscious mice. Am J Physiol Renal Physiol. 2012;303(5):F783-F788. doi:10.1152/ajprenal.00279.2012
   (cite for procedure and mouse conversion factor)

2. Scarfe L, Schock-Kusch D, Ressel L, Friedemann J, Shulhevich Y, Murray P, Wilm B, de Caestecker M. Transdermal Measurement of Glomerular Filtration Rate in Mice. Vis. Exp. (140), e58520, doi:10.3791/58520 (2018). (cite for procedure)

3. Schock-Kusch D, Sadick M, Henninger N, Kraenzlin B, Claus G, Kloetzer H-M, Weiß C, Pill J, Gretz N. Transcutaneous measurement of glomerular filtration rate using FITC-sinistrin in rats, Nephrology Dialysis Transplantation, Volume 24, Issue 10, October 2009, Pages 2997–3001, https://doi.org/10.1093/ndt/gfp225 (cite for rat conversion factor)

4. Friedemann J, Heinrich R, Shulhevich Y, Raedle M, William-Olsson L, Pill J, Schock-Kusch D. Improved kinetic model for the transcutaneous measurement of glomerular filtration rate in experimental animals. Kidney Int. 2016 Dec;90(6):1377-1385. doi: 10.1016/j.kint.2016.07.024. Epub 2016 Sep 21. PMID: 27665115. (cite for 3 compartment model used in MB Studio software)